Fig. 4

Subcellular localization of subclass III SnRK2 proteins. (A) Schematic illustration of the constructs. (B) The construct containing ZmSnRK2s:GFP fusion protein vector into the Agrobacterium tumefaciens strain GV3101 and used to infect Nicotiana benthamiana leaf cell. The bars, 50 μm. (C) The ZmSnRK2s:GFP fusion protein was transformed into maize leaf protoplasts respectively. The bars, 10 μm. All fluorescence signals were detected using the Confocal microscope. 35S-eGFP was used as the control. Merged, individual images of GFP and bright field images of onion epidermal cells were shown. GFP, green fluorescent protein. The localization of the nuclei was indicated by DAPI (6-diamidino-2-phenylindole) staining