Fig. 2

Expression patterns of genes related to the isoflavonoid biosynthesis pathway. a Schematic representation of the isoflavonoid biosynthesis pathway in legumes. b Differentially expressed genes (DEGs) encoding proteins related to isoflavonoid biosynthesis in control and PvRbohB-RNAi P. vulgaris roots at 7 dpi with R. tropici. The color bars represent the log₂(fold change), with red representing upregulated genes and blue representing downregulated genes. A cut-off threshold of log₂FC ≥ 1.5, Padj/FDR ≤ 0.05 was used. PAL: phenylalanine ammonia-lyase; C4H: cinnamate 4-hydroxylase; 4CL: 4-coumarate CoA-ligase; CHS: chalcone synthase; CHR: chalcone reductase; CHI: chalcone isomerase; IFS: isoflavone synthase; CYP81E: isoflavone/4'-methoxyisoflavone 2'-hydroxylase