Fig. 8
Concentrations of free asparagine in the grain of different varieties of wheat in which the TaASN-B2 gene was either present or absent in two field trials, 2011–2012 and 2012–2013, showing the effect of the TaASN-B2 deletion under different sulphur treatments (S- and S+) and the interaction between the presence/absence of the gene and treatment. (a) Back-transformed data from the 2011–2012 and 2012–2013 trials with concentrations from the S- and S+ treatments plotted together. (b) Back-transformed data from the 2011–2012 season with concentrations from the S- and S+ treatments plotted separately. (c) Back-transformed data from the 2012–2013 season with concentrations from the S- and S+ treatments plotted separately. (d) Back-transformed predicted means from the REML analysis for the 11 varieties grown in both trials. Crosses indicate the mean of each group. Boxes show the interquartile range and median. Whiskers show the smallest and largest value within 1.5 times of the interquartile range above and below the 75th and 25th percentile, respectively. Significance scores for the main effect of TaASN-B2 in (a) were taken from separate ANOVA analyses performed on loge transformed data. Multiple comparisons with Bonferroni correction were performed following the ANOVA analyses for TaASN-B2 group comparisons in 2011–2012 (b) and 2012–2013 (c). NS = p > 0.05, ** = p < 0.01, *** = p < 0.001