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Fig. 7 | BMC Plant Biology

Fig. 7

From: Reduced free asparagine in wheat grain resulting from a natural deletion of TaASN-B2: investigating and exploiting diversity in the asparagine synthetase gene family to improve wheat quality

Fig. 7

Expression analysis by RT-qPCR of the TaASN2 homeologues in the embryo of four varieties of wheat either possessing the TaASN-B2 gene (Cadenza (a.) and Duxford (b.)) or lacking one (Claire (c.) and Spark (d.)) at 14-, 21-, and 28-days post anthesis (DPA). Expression levels are relative to three reference genes (GAPDH, PROSM, and SDH). RE (relative expression), E (PCR efficiency calculated by LinRegPCR), Ct (threshold cycle). Error bars show standard error of the mean

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