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Fig. 2 | BMC Plant Biology

Fig. 2

From: The transcription factor AtGLK1 acts upstream of MYBL2 to genetically regulate sucrose-induced anthocyanin biosynthesis in Arabidopsis

Fig. 2

Anthocyanin accumulation in wild-type, single mutants of glk1 and glk2, and the glk1 glk2 double mutant. a Real-time quantitative PCR analysis of the AtGLK1 and the AtGLK2 transcript accumulation in the wild type (Col), single mutants of glk1 and glk2, and the glk1 glk2 double mutant seedlings. The total RNA was isolated from 4-d-old seedlings grown on 1/2 MS medium supplemented with 2% sucrose. b Images of representative seedlings of the wild-type (Col), single mutants of glk1 and glk2, and the glk1 glk2 double mutant grown for 4 days on 1/2 MS medium supplemented without sucrose (-S), with 2% sucrose (+ S), or with 58 mM mannitol (Man), respectively. The black arrows indicate the locations of the anthocyanin accumulation in different genotypic Arabidopsis seedlings. c Quantitative measurement of anthocyanins in 4-d-old seedlings (Col, glk1, glk2 and glk1 glk2) grown on 1/2 MS medium supplemented without sucrose (-S), with 2% sucrose (+ S), or with 58 mM mannitol (Man), respectively. The asterisks indicate statistically significant differences compared with the corresponding wild-type (Student’s t test: *P < 0.05)

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