Fig. 3
Auto fluorescence from toluidine blue stained sections of skins types seen in the backcross population. Scale bars represent 50 μM. A Epidermal skins with weakly fluorescing cuticle; B skins with russeted suberized cells and trichomes; C and D skins with russeted suberized cells; D russeted suberized cells covered with lignified cells. Suberin can be identified by bright blue fluorescence. Blue-green auto fluorescence indicates the presence of lignin. Auto fluorescence that occurred as a bright blue single layer above the L1 epidermal layer was taken as fluorescence of the cuticle