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Table 8 Effect of chilling stress on coriander (Coriandrum sativum L.) seeds pre- soaked in 80 mM pot. Silicate, 50 mg l−1 humic acid or soaked in water after exposure to γ-rays (50 Gy) and the interaction of the alleviation elements and chilling stress on antioxidant enzymes PPO, POD, CAT (unit/mg protein) and MDA(nmol/g F. wt.) at flowering stage. The shown data was extracted by using 3 biological and 3 technical replicates. Each biological replicate comprised of 10 plants (one pot). To perform the biochemical analysis, the combined tissue of these ten plants refers to one technical replicate. Sample extraction was done solely for each technical replicate. The readings of the 3 technical replicates were recorded. The mean of the values was used to calculate ±SE. Also, the least significant differences (LSD) at 5% level were calculated to compare the means of different treatments according to Snedecor and Cochran [92]. The values with the same letter are not significantly different (P<0.05). The raw data set of the technical replicates was attached as a supplementary file. The percentage of increase (inc.) caused by the chilling stress compared with the control values was investigated. By being the most alleviation element to restore quietly the enzymes steady state concentration (except for POD and MDA), the percentage of decrease compared with the chilling stress values in all measurements triggered by HA application was further investigated. This percentage was calculated by subtracting the value of control/chillied reading from the reading value of any physiological treatment, then the result was divided by the reading of control value, and finally, the result is multiplied by 100

From: Metabolic activities and molecular investigations of the ameliorative impact of some growth biostimulators on chilling-stressed coriander (Coriandrum sativum L.) plant

Antioxidant enzyme/MDA Antioxidants enzyme (unit/mg protein) Lipid peroxidation (nmol/g F. wt.)
Treatment
PPO POD CAT MDA
Control 5589.2 ± 115cd 1.49 ± 0.12e 0.604 ± 0.06c 0.481 ± 0.01e
Chilling (6 °C ± 0.5) 8966.5 ± 577a 5.23 ± 0.12a 1.24 ± 0.1a 0.888 ± 0.03a
Pot. Silicate (80 mM) 6821.5 ± 58.9b 3.08 ± 0.12bcd 0.952 ± 0.19b 0.582 ± 0.02cd
Humic acid (50 mg l−1) 4608.5 ± 86ef 3.88 ± 0.42b 0.418 ± 0.1cd 0.642 ± 0.02bc
γ-rays (50 Gy) 4059.4 ± 209f 2.95 ± 0.12cd 0.212 ± 0.002d 0.558 ± 0.019de
Chilling + Pot. silicate 6226.6 ± 101bc 3.48 ± 0.69bc 0.692 ± 0.1bc 0.703 ± 0.027b
Chilling + Humic acid 4996.4 ± 288de 3.21 ± 0.12bcd 0.596 ± 0.02c 0.702 ± 0.024 b
Chilling + γ-rays 8234.01 ± 126a 2.44 ± 0.43d 0.212 ± 0.002d 0.572 ± 0.026cd
LSD at 0.05 758 0.8964 0.2769 0.078
% of inc. in chilling values compared with control 60.4 251 106 84.61
% of dec. in Chilling + HA compared chilled plants 44.3 38.6 51.93 20.94