Fig. 2

Immunoblot analysis of the expression of chloroplast marker proteins. Coriander control (Lane 1) and chilling-stressed (Lane 2) ones were subjected to combination of chilling stress in presence of 80 mM Pot. silicate (Lane 3), 50 mg l^− 1 HA (Lane 4) or soaked in water after exposed to 50 Gy gamma irradiation (Lane 5). TCPs were extracted and fractionated by SDS-PAGE and immunodecorated against α-Toc34, α-Toc75, eHSP70, and actin primary antibody in a dilution of 1:10,000 as demonstrated in [51]. Cropping of the shown blots was performed properly for sake of clarity and focusing the information. Full-length blots are accompanied the manuscript as Supplementary Fig. 3. Protein extraction procedure for each physiological status (control, chilling stressed, etc.) was performed from the leaves of 3 biological replicates and 3 technical replicates. Each technical replicate represented one biological replicate. Each biological replicate comprises the collection of leaves of 10 plants. The protein extraction was carried out from each technical replicate independently. Finally extracted proteins from the 3 technical replicates were pooled together. Pooled sample were quantified, equally loaded into 10% SDS-PAGE, and blotted onto PVDF membrane as shown in methods section. Consequently, aliquots of pooled sample were kept as −80 °C after the short snap for 30 s in Liquid Nitrogen