Two β-glucuronosyltransferases involved in the biosynthesis of type II arabinogalactans function in mucilage polysaccharide matrix organization in Arabidopsis thaliana

Table 2 Total sugar estimation (μg/mg of seed) in WT and glcat14 mutants

Extracts^a	WT	glcat14a-1	glcat14c-1	glcat14a-1glcat14c-1
Ammonium oxalate	5.74 ± 0.46	6.03 ± 0.35	5.97 ± 0.12	7.80 ± 0.22*
0.2 N NaOH	11.73 ± 0.63	13.9 ± 0.42*	12.94 ± 0.83	15.04 ± 0.42*
2 N NaOH	10.59 ± 0.72	9.6 ± 0.32	10.23 ± 0.29	4.39 ± 0.33*
Total Sugar	28.06	29.53	29.14	27.23

^aQuantification of total sugars from wild type and glcat14 mutants mucilage sequentially extracted using 0.2% ammonium oxalate, 0.2 N NaOH and 2 N NaOH neutralized and assayed with the phenol–sulfuric acid method against glucose standards. Results are given as µg/mg seed ± SE. Significant differences from wild type (WT), (P < 0.05) are indicated with an asterisk and shown in bold

ISSN: 1471-2229