Skip to main content

Table 2 Total sugar estimation (μg/mg of seed) in WT and glcat14 mutants

From: Two β-glucuronosyltransferases involved in the biosynthesis of type II arabinogalactans function in mucilage polysaccharide matrix organization in Arabidopsis thaliana

Extractsa WT glcat14a-1 glcat14c-1 glcat14a-1glcat14c-1
Ammonium oxalate 5.74 ± 0.46 6.03 ± 0.35 5.97 ± 0.12 7.80 ± 0.22*
0.2 N NaOH 11.73 ± 0.63 13.9 ± 0.42* 12.94 ± 0.83 15.04 ± 0.42*
2 N NaOH 10.59 ± 0.72 9.6 ± 0.32 10.23 ± 0.29 4.39 ± 0.33*
Total Sugar 28.06 29.53 29.14 27.23
  1. aQuantification of total sugars from wild type and glcat14 mutants mucilage sequentially extracted using 0.2% ammonium oxalate, 0.2 N NaOH and 2 N NaOH neutralized and assayed with the phenol–sulfuric acid method against glucose standards. Results are given as µg/mg seed ± SE. Significant differences from wild type (WT), (P < 0.05) are indicated with an asterisk and shown in bold