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Table 1 Monosaccharide composition analysis of WT and glcat14 mutants

From: Two β-glucuronosyltransferases involved in the biosynthesis of type II arabinogalactans function in mucilage polysaccharide matrix organization in Arabidopsis thaliana

Non-Adherent mucilage (mol %) Adherent mucilage (mol%) Whole Seed (mol%)
Sugar Wild Type glcat14a-1 glcat14c-1 glcat14a glcat14c Wild Type glcat14a-1 glcat14c-1 glcat14a glcat14c Wild Type glcat14a-1 glcat14c-1 glcat14a glcat14c
Fuc 1.31 ± 0. 06 1.12 ± 0.02 1.05 ± 0.03 0.44 ± 0.01* 0.71 ± 0.04 0.82 ± 0.02 0.65 ± 0.04 2.66 ± 0.01** 2.02 ± 0.13 1.92 ± 0.14 1.62 ± 0.53 1.79 ± 0.36
Rha 31.53 ± 0.51 28.61 ± 0.17 27.44 ± 0.41 29.2 ± 0.39** 25.58 ± 0.43 27.85 ± 0.69 28.47 ± 0.41 26.63 ± 0.22** 17.03 ± 0.25 18.42 ± 0.24 18.03 ± 0.27 16.67 ± 0.54
Ara 0.38 ± 0.01 0.34 ± 0.05 0.29 ± 0.08 0.15 ± 0.01** 0.09 ± 0.012 0.12 ± 0.013 0.18 ± 0.015 0.60 ± 0.01** 20.59 ± 0.13 19.37 ± 0.37 20.24 ± 0.67 19.61 ± 0.46
Gal 3.68 ± 0.09 3.59 ± 0.09 4.97 ± 0.08 2.74 ± 0.05* 5.97 ± 0.02 6.09 ± 0.11 5.78 ± 0.04 13.20 ± 0.07** 15.76 ± 0.14 15.39 ± 0.45 15.81 ± 0.26 16.77 ± 0.32
Glc 3.80 ± 0.01 3.92 ± 0.03 3.63 ± 0.02 2.28 ± 0.06* 5.87 ± 0.07 5.98 ± 0.06 6.36 ± 0.05 10.85 ± 0.06** 4.69 ± 0.12 5.16 ± 0.25 4.65 ± 0.12 6.72 ± 0.36*
Xyl 7.95 ± 0.01 7.55 ± 0.02 5.53 ± 0.02 5.4 ± 0.05* 4.8 ± 0.06 5.43 ± 0.22 4.55 ± 0.03 7.66 ± 0.08** 12.76 ± 0.23 12.03 ± 0.66 12.39 ± 0.37 13.04 ± 0.83
Man 3.42 ± 0.04 3.03 ± 0.03 2.68 ± 0.04 1.20 ± 0.03* 3.23 ± 0.25 2.89 ± 0.36 2.84 ± 0.17 6.53 ± 0.11** 2.76 ± 0.25 2.11 ± 0.11 2.00 ± 0.39 2.38 ± 0.82
GalA 47.59 ± 0.13 51.55 ± 0.68 54.15 ± 0.73* 58.55 ± 0.92** 53.7 ± 0.35 50.81 ± 0.67* 51.17 ± 0.98 31.87 ± 0.11** 24,39 ± 0.16 25.60 ± 0.45 25.25 ± 0.61 23.02 ± 0.69
GlcA 0.34 ± 0.01 0.30 ± 0.03 0.26 ± 0.03 0.04 ± 0. 01** 0.05 ± 0.01 n.d n.d n.d     
  1. *P < 0.05; **P < 0.01; n.d Not detected; Sugars significantly different from WT are indicated in bold