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Fig. 5 | BMC Plant Biology

Fig. 5

From: Two β-glucuronosyltransferases involved in the biosynthesis of type II arabinogalactans function in mucilage polysaccharide matrix organization in Arabidopsis thaliana

Fig. 5

Cellulose deposition is altered in glcat14a-1 glcat14c-1 double mutants. a Pontamine fast scarlet (S4B) cellulose staining of the adherent mucilage of water hydrated mature seeds (Upper panel) and EDTA hydrated seeds (lower panel). b Calcofluor staining of the adherent mucilage of WT, glcat14 single and double mutant seeds. Immunolabelling of CBM3a (c) with high affinity to crystalline cellulose in adherent mucilage and CBM28 (d) were counterstained with the S4B dye. Visualization of polarized light birefringence by crystalline cellulose in adherent mucilage released from WT and mutant seeds (e). Quantification of crystalline cellulose contents in whole seeds, demucilaged seeds, and in the mucilage of WT and mutants (f) using the Updegraff assay. Values represents the means ± SD of 4 biological replicates. The single asterisk marks a significant decrease compared with WT (Student’s t-test, P < 0.05 for single asterisks). Bars = 100 μm

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BMC Plant Biology

ISSN: 1471-2229

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