Skip to main content
Fig. 5 | BMC Plant Biology

Fig. 5

From: RUS6, a DUF647-containing protein, is essential for early embryonic development in Arabidopsis thaliana

Fig. 5

RUS6 is essential for normal embryonic development. Embryos dissected out from seeds of both rus6-1/ + and WT plants were examined by differential interference contrast (DIC) microscopy. Shapes of the observed embryo are traced with dotted lines to outline the positions of the embryos. Images of embryos from the same rus6-1/ + silique demonstrate that embryos from white seeds are severely developmentally delayed (~ 10 × smaller) as compared to those from green seeds, and fail to develop past the mid- to late-globular phase. a-b Defective embryos from white seeds stalled at the mid- to late-globular phase show a distorted/absent suspensor, a lack of identifiable columella, and initial stages of deterioration. c A white seed from a more mature rus6-1/ + silique has no detectable embryo. d An arrested embryo from a white seed in mid-globular phase that has not yet begun to deteriorate. e A representative transition to early heart stage embryo from a WT silique. No rus6-1/ + white seed embryos were observed at this phase. f A representative late-bent-cotyledon stage embryo from a green seed shows normal development as compared to white seeds in the same rus6-1/ + silique shown in a, b, and d. Bar = 5 microns (a, b, d, and e). Bar = 50 microns (c and f)

Back to article page