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Fig. 4 | BMC Plant Biology

Fig. 4

From: RUS6, a DUF647-containing protein, is essential for early embryonic development in Arabidopsis thaliana

Fig. 4

Complementation of homozygous rus6-1 mutants. rus6-1/ + plants were transformed with a RUS6::RUS6-GFP construct, and homozygous rus6-1 plants carrying RUS6::RUS6-GFP (Compl) were recovered. a Images of 7-days-old seedlings of WT (WT), the complementation line (Compl) (in homozygous rus6-1 background), and the rus6-1/ + line, are shown. Bar = 0.5 cm. b The complementation line carrying RUS6::RUS6-GFP is rus6-1 homozygous. Gel images of specific markers for T-DNA detection (T-DNA detection), WT RUS6 (WT RUS6 detection), and the RUS6::RUS6-GFP transgene (RUS6-GFP detection) are shown. 1 kb: 1 kb DNA ladder. kb: kilobase. c RUS6::RUS6-GFP rescues the rus6-1 white seed phenotype. Image of a representative dissected silique from the RUS6::RUS6-GFP complemented line (in rus6-1 homozygous background) is shown. Bar = 1 mm

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