Fig. 1

Identification and molecular characterization of T-DNA knockouts for RUS3, RUS4 and RUS5. a T-DNA insertion position in the RUS3 gene (AT1G13770) was detected and confirmed in the SALK_135717C T-DNA line. b T-DNA insertion position in the RUS4 (AT2G23470) gene was detected and confirmed in GK-447F02 T-DNA line. c T-DNA insertion position in the RUS5 gene (AT5G01510) was detected and confirmed in SALK_038772 T-DNA line. Filled boxes indicate exons. Positions for the start (ATG) and stop codons (TGA in RUS3; TAA in RUS4 and RUS5) are indicated. Positions of the primer pairs used to detect either the presence of the T-DNA insertions or amplify the wild-type genomic sequence are indicated. Gel images of DNA fragments amplified with pairs of primers as indicated (Primer pair) below the gel image, from either the corresponding T-DNA line or the WT (wild type) are shown at the right side. Sizes of DNA ladder (1 kb) bands are indicated in kb (kilobase)