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Table 2 Expression of the genes in the early axillary meristem tissues and effects of RNA interference

From: Inhibition of lateral shoot formation by RNA interference and chemically induced mutations to genes expressed in the axillary meristem of Nicotiana tabacum L.

Gene

RNAseq counts (Reads Per Kilobase of exon per Million mapped sequence reads)

Effect of RNAi measured relative to null segregants ± SD

RNA expression

Primary lateral shoots

Secondary lateral shoots

EA

VE

Control

Number

Weight

Number

Weight

NtLs

1.16

62.97

1.14

0.50 ± 0.08

1.00 ± 0.00

1.28 ± 0.15

0.13 ± 0.09a

0.1 ± 0.06a

NtBl1

12.80

108.85

8.43

0.31 ± 0.05

1.02 ± 0.06

1.21 ± 0.18

0.00 ± 0.00a

0.00 ± 0.00a

NtBl2

ND

ND

ND

0.19 ± 0.04

1.00 ± 0.00

1.13 ± 0.15

1.00 ± 0.10

1.46 ± 1.65

NtBl3

11.88

23.20

10.26

0.30 ± 0.09

1.00 ± 0.00

1.45 ± 0.48

0.88 ± 0.46

0.77 ± 0.12

NtREV

110.74

167.99

305.61

0.62 ± 0.10

1.00 ± 0.00

0.98 ± 0.09

0.06 ± 0.10a

0.03 ± 0.04a

NtCUC1

4.24

10.32

8.38

0.16 ± 0.07

1.00 ± 0.00

1.05 ± 0.12

0.91 ± 0.18

0.57 ± 0.10

NtCUC2

ND

ND

ND

0.53 ± 0.04

1.00 ± 0.00

1,14 ± 0.26

0.97 ± 0.12

1.06 ± 0.4

NtCUC3

ND

ND

ND

0.17 ± 0.10

1.00 ± 0.00

0.96 ± 0.17

1.08 ± 0.01

0.71 ± 0.26

NtCUC4

ND

ND

ND

0.69 ± 0.03

1.00 ± 0.00

0.91 ± 0.04

1.05 ± 0.09

0.75 ± 0.19

NtFHY3

23.49

36.90

46.59

0.31 ± 0.17

1.00 ± 0.00

0.94 ± 0.08

1.03 ± 0.11

1.12 ± 0.62

NtLOM1

36.69

49.15

65.87

0.38 ± 0.06

1.00 ± 0.00

1.09 ± 0.15

1.72 ± 1.97

2.56 ± 3.99

NtLOF1

30.27

60.57

25.56

0.38 ± 0.11

1.01 ± 0.02

1.67 ± 0.15

0.28 ± 0.30

0.64 ± 0.58

EA1

32.93

2.30

ND

0.09 ± 0.03

1.00 ± 0.00

1.14 ± 0.16

0.97 ± 0.15

1.21 ± 0.34

EA2

20.13

9.70

1.48

0.28 ± 0.16

1.00 ± 0.00

1.17 ± 0.35

1.34 ± 0.82

1.65 ± 1.59

EA3

22.56

1.77

ND

0.16 ± 0.03

1.00 ± 0.00

0.93 ± 0.09

0.68 ± 0.16

1.42 ± 1.53

EA4

43.22

2.50

1.19

0.35 ± 0.17

1.00 ± 0.00

1.16 ± 0.28

1.14 ± 0.41

1.09 ± 0.38

EA5

14.41

ND

ND

0.10 ± 0.04

1.00 ± 0.00

1.04 ± 0.07

4.05 ± 2.21

18.88 ± 28.34

EA6

22.78

ND

ND

0.80 ± 0.22

1.00 ± 0.00

1.01 ± 0.26

1.04 ± 0.27

1.30 ± 0.80

EA7

12.14

ND

ND

0.10 ± 0.01

1.00 ± 0.00

1.02 ± 0.17

0.83 ± 0.76

0.77 ± 0.70

EA8

43.57

ND

1.47

0.02 ± 0.02

1.00 ± 0.00

1.08 ± 0.36

1.14 ± 0.11

1.21 ± 0.2

EA9

14.03

ND

ND

0.17 ± 0.12

1.00 ± 0.00

0.95 ± 0.17

1.10 ± 0.77

1.16 ± 0.74

EA10

51.93

8.72

1.25

0.43 ± 0.08

1.00 ± 0.00

1.33 ± 0.16

1.59 ± 0.99

0.96 ± 0.21

EA11

19.63

1.70

1.62

0.56 ± 0.08

1.00 ± 0.00

0.98 ± 0.27

0.98 ± 0.09

1.52 ± 0.92

VE1

39.71

49.37

3.79

0.58 ± 0.06

1.00 ± 0.00

0.99 ± 0.30

1.10 ± 0.16

1.27 ± 0.51

VE2

26.70

32.21

0.69

0.51 ± 0.02

1.00 ± 0.00

1.04 ± 0.12

1.09 ± 0.17

0.95 ± 0.54

VE3

6.51

49.14

ND

1.51 ± 0.05

1.00 ± 0.00

1.16 ± 0.21

1.02 ± 0.45

0.97 ± 0.35

VE4

ND

10.94

ND

0.32 ± 0.04

1.00 ± 0.00

1.27 ± 0.31

0.80 ± 0.38

0.95 ± 0.64

VE5

20.88

79.27

3.12

0.41 ± 0.06

1.00 ± 0.00

1.00 ± 0.17

1.3 ± 0.74

1.41 ± 0.84

VE6

2.61

31.26

ND

0.35 ± 0.14

1.00 ± 0.00

0.73 ± 0.12

2.06 ± 0.56

4.57 ± 3.20

VE7

ND

73.71

4.97

0.08 ± 0.02

1.00 ± 0.00

1.49 ± 0.30

0.33 ± 0.09a

0.26 ± 0.11a

VE8

5.93

13.38

ND

0.14 ± 0.06

1.00 ± 0.00

1.01 ± 0.28

1.41 ± 0.43

0.87 ± 0.39

VE9

5.06

14.07

ND

0.62 ± 0.08

1.00 ± 0.00

0.98 ± 0.10

1.03 ± 0.16

0.90 ± 0.12

VE10

9.42

21.58

1.86

0.42 ± 0.04

1.00 ± 0.00

0.97 ± 0.25

1.16 ± 0.62

1.79 ± 1.29

VE11

ND

13.62

ND

0.70 ± 0.07

1.00 ± 0.00

1.07 ± 0.28

0.58 ± 0.56

0.42 ± 0.43

VE12

19.79

133.95

11.71

0.18 ± 0.06

1.00 ± 0.00

1.22 ± 0.12

0.29 ± 0.41

0.05 ± 0.08a

VE13

5.68

19.24

ND

0.14 ± 0.05

1.00 ± 0.00

1.03 ± 0.21

1.03 ± 0.12

1.86 ± 0.94

  1. ND Not detectable
  2. aSignificantly different from null segregants at the 1% level as determined by the t-test, assuming that the means of the relative values were from normal distributions