Fig. 5

Moderate N stress is sufficient to repress the low P response. a Representative 25-day-old maize seedlings grown across five levels of N availability (N5 to N1, high to low) and two levels of P availability (P5, high and P1, low). b Shoot fresh weight of maize seedlings grown as A. Boxes show 1st quartile, median and 3rd quartile of 4 biological replicates. Whiskers extend to the most extreme points within 1.5x box length; outlying values beyond this range are not shown. Letters indicate groups based on HSD Tukey (p < 0.05). Transcript accumulation (relative abundance) determined by real-time PCR for c leaves and d roots of 25-day-old maize seedlings grown as A. Median of 5 biological replicates. Pap10 - Purple acid phosphatase10, GRMZM2G093101; Pht1;9 - Phosphorus transporter1;9, GRMZM2G154090; Pht1;13 - Phosphorus transporter1;13, GRMZM2G070087; Mfs2 - ZmSPX-MFS2, GRMZM2G166976; Npf6.6 - Nitrate/peptide Transporter6.6, GRMZM2G161459); Nir-a - nitrite reductase-a, GRMZM2G079381)