Fig. 5

The BxSCD5 candidate effector of Bursaphelenchus xylophilus suppresses PAMP PsXEG1-triggered cell death in Nicotiana benthamiana. a Representative N. benthamiana leaves at 7 days after inoculation with Agrobacterium sp. strain GV3101 carrying five common candidate effectors [with and without native signal peptide (SP)] of B. xylophilus in the pGR107 vector. Ratios in the picture indicate the proportion of infiltrated sites that developed the cell death phenotype. The assay was repeated at least three times. In each assay, three different plants with three inoculated leaves were used, yielding similar results. b Functional detection of five common candidate effectors (with and without native SP) of B. xylophilus to inhibit PAMP PsXEG1-triggered cell death. Ratios in the picture indicate the proportion of infiltrated sites that developed the cell death phenotype. The infiltration assay was performed three times, and in each assay, three different plants with three inoculated leaves were used, yielding similar results