Fig. 4

The expression pattern of the five common candidate effectors from the three transcriptome datasets for Bursaphelenchus xylophilus at the early infection stages by quantitative real-time polymerase chain reaction (qRT-PCR) analysis. Relative expression of the five candidate effectors at the mycetophagous stage (0 h) and at the early stages of infection (2.5, 6, 12 and 24 h). Data represent the means, and the error bars represent ± SD from three biological replicates. Different letters on top of the bars indicate statistically significant differences (p < 0.05, t test) as measured by Duncan’s multiple range test