Fig. 6

Silencing of GhWAKs increased the spread of V. dahliae in the cotton stems. a, Detection for V. dahliae in cotton stems at 5 dpi and 7 dpi by PCR. M, marker. DNA templates for PCR were extracted from V. dahliae spores as positive control (lane 1), water as negative control (lane 2), and cotton seedling stems (lane 3–18). b, Isolation of V. dahliae from the stems of GhWAK26-silenced and GhWAK77-silenced cotton plants by PDA cultivation. Bars = 0.5 cm. c, The lignin content in GhWAK-silenced plants and CK. The results from three biological replicates are shown with mean ± SE. Asterisks represent P values (***P < 0.001; Dunnett’s multiple comparisons test)