Fig. 4
Expression of GhWAKs in response to V. dahliae infection. a, Heatmap representation for the expression patterns of 26 GhWAKs differentially expressed as a result of cotton inoculation with V. dahliae, compared to the respective control. Expression levels of genes were shown as the Log2 Fold change (FC). FC is a ratio of treatment FPKM to control FPKM that obtained from the RNA-Seq data. Higher and lower transcriptional level are indicated by pink and blue, respectively, and no detected expression is indicated by dark color. b and c, The expression analysis of GhWAKs from G. hirsutum ND601 induced with V. dahliae by qRT-PCR. The relative gene expression level was calculated using the comparative 2-ΔΔCt method with GhHis3 as internal control. The bar represents the standard error (SE) calculated from three independent experiments. Asterisks indicate statistically significant differences according to Sidak’s multiple comparisons test (**P < 0.001, ***P < 0.01; ns, no significant)