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Fig. 7 | BMC Plant Biology

Fig. 7

From: Genome-wide identification, evolutionary estimation and functional characterization of two cotton CKI gene types

Fig. 7

Expression profiles of two types G. hirsutum CKI genes in anthers at different development stages under NT and HT conditions. a-b Expression patterns of G. hirsutum CKI genes in 84,021 and H05 at different anther developmental stages under NT and HT conditions from RNAseq (a) and Quantitative RT-PCR experiment (b). 8 N and 8H refer to 84,021 (the HT-tolerant line) under NT and HT conditions, respectively; HN and HH refer to H05 (the HT-sensitive line) under NT and HT conditions, respectively; TS: tetrads stage; TDS: tapetal degradation stage; ADS, anther dehiscence stage; FPKM: fragments per kb of transcript per million fragments mapped. The RNAseq data of the expression profiles was cited from Min et al. [33]. Error bars indicate ± standard deviations of triplicate experiments. Asterisks indicate statistically significant differences (*P < 0.05, **P < 0.01) by Student’s t-test. The GhUB7 (Gh_A11G096) was used as the reference gene to normalize the total amount of cDNA in each reaction

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