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Fig. 7 | BMC Plant Biology

Fig. 7

From: Inter-species functional compatibility of the Theobroma cacao and Arabidopsis FT orthologs: 90 million years of functional conservation of meristem identity genes

Fig. 7

AtFT transgenic and PSU-Sca6 control cacao pollen viability assessed by in vitro germination. The germination rate for control PSU-Sca6 pollen was assayed at a range of experimental conditions. a and e Control pollen germinated optimally with flowers incubated at 28 °C pre-test and pollen tested at 26 °C. Red arrows in (a) highlight the consistency with which this experimental regime led to higher germination rates even in unfavorable media compositions. (A-D) AtFT pollen germinated at a similar average rate as pollen from control flowers assayed under similar conditions. b-c Micrographs of AtFT pollen in vitro germination; tested transgenic pollen displayed diverse germination rates as pictured. d Micrograph of control pollen in vitro germination in experimental conditions: media A, pre-test incubation of 23 °C, pollen assay at 23 °C. e Micrograph of control pollen germination at optimal experimental conditions. Pollen tubes in (e) are markedly longer than tubes in (b-d). Media compositions are listed in Table S4. °C—°C temperature in (a) legend indicate pre-test and pollen germination temperatures, respectively

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