Table 2 Genetic analyses of T-DNA segregation in the T1 population of two constructs
Event ID | Construct | Detection of RFP expression | Taqman assay | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. seeds RFP+ | No. seeds RFP- | Transgene-free % | Observed ratio | Expected ratio | χ2 | T-DNA + | T-DNA - | Transgene-free % | Observed ratio | Expected ratio | χ2 | Ave. accuracy rate of visual selection | ||
L18 | 24277 | 103 | 97 | 48.50% | 1: 0.94 | 1: 1 | 0.18 | 69 | 61 | 46.92% | 1: 0.88 | 1: 1 | 0.49 | 98.62% |
L31 | 98 | 103 | 51.24% | 1: 1.05 | 1: 1 | 0.12 | 96 | 101 | 51.27% | 1: 1.05 | 1: 1 | 0.13 | ||
L33 | 98 | 102 | 51.00% | 1: 1.04 | 1: 1 | 0.08 | 96 | 101 | 51.27% | 1: 1.05 | 1: 1 | 0.13 | ||
L5 | 98 | 103 | 51.24% | 1: 1.05 | 1: 1 | 0.12 | 93 | 99 | 51.56% | 1: 1.06 | 1: 1 | 0.19 | ||
L4 | 93 | 107 | 53.50% | 1: 1.15 | 1: 1 | 0.98 | 92 | 106 | 53.54% | 1: 1.15 | 1: 1 | 0.99 | ||
L8 | 24259 | 146 | 44 | 23.16% | 3.30: 1 | 3: 1 | 0.34 | N/A | ||||||
L21 | 148 | 48 | 24.49% | 3.08: 1 | 3: 1 | 0.03 | ||||||||
L22 | 151 | 37 | 19.68% | 4.08: 1 | 3: 1 | 2.84 | ||||||||
L26 | 143 | 51 | 26.29% | 2.80: 1 | 3: 1 | 0.17 | ||||||||
L34 | 140 | 59 | 29.65% | 2.37: 1 | 3: 1 | 2.29 | ||||||||