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Fig. 1 | BMC Plant Biology

Fig. 1

From: Highly efficient generation of bacterial leaf blight-resistant and transgene-free rice using a genome editing and multiplexed selection system

Fig. 1

Schematic diagram of the strategy for transgene-free plant enrichment and selection. Increasing the proportion of nontransgenic offspring by hampering T-DNA transmission in male gametophytes and sorting nontransgenic seeds by using a visual selection marker. For single T-DNA insertion lines, self-pollination of the resulting T1 propagates 50% of the transgenic seeds and 50% of the nontransgenic seeds, indicating that the proportion of desired T1 seeds will be increased from 25 to 50%. For multicopy T-DNA insertion lines, the T-DNA-free progeny segregation rate will increase 2n (2 to the power n) times. N is equal to the copy number

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