Fig. 6
From: Simultaneous gene expression and multi-gene silencing in Zea mays using maize dwarf mosaic virus
Aphid transmission of virus launched from pWX56 vs. wild type control pWX6. A total of 20 plants each for pWX6 and pWX56 were aphid inoculated with the respective constructs, and infectivity was scored by observation of mosaic symptoms and RT-PCR analysis (data not shown). No statistical significance was observed between pWX6 and pWX56 transmission or between transmission by Kansas and Ohio-derived R. padi colonies. Samples were taken from the newest fully emerged leaf of 17 dpi of aphid feeding plants for RT-PCR analysis using primers WX358/WX367 (1115 nt) for GFP insertion, and primers WX317/WX315 (965 nt) for VIGS insertion