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Fig. 2 | BMC Plant Biology

Fig. 2

From: Simultaneous gene expression and multi-gene silencing in Zea mays using maize dwarf mosaic virus

Fig. 2

Images of GFP visualization and symptoms by MDMV OH5 constructs at 7 and 21-days post inoculation on Z. mays cultivar Silver Queen. a. pWX27. b. pWX68. c. pWX56. d. Non-inoculated control. e. Non-inoculated maize. f. pWX56-infected plant leaf with virus-induced gene silencing photobleaching. g. pWX6, a wild-type clone of MDMV OH5. The newest fully emerged leaf from top of the plants were selected for GFP visualization. a-d. Fluorescent images taken with a Leica DFC460C camera using fluorescence imaging with NIGHTSEA Green-only bandpass filter at 3 s exposure. Brightfield images using same camera without fluorescence at 1 s exposure. e-g. Z. mays ‘Silver Queen’ plant images at 21 dpi taken with a Canon EOS REBEL T5i

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