Fig. 1
From: Simultaneous gene expression and multi-gene silencing in Zea mays using maize dwarf mosaic virus
Genome layout of MDMV OH5 full-length infectious clone constructs. a. pWX6 (14,229 bp), infectious wild-type clone. b. pWX27 (15,009 bp), GFP gene inserted in-frame between NIb and CP coding sequences. c. pWX68 (15,018 bp), GFP gene inserted in-frame between P1 and HCPro coding sequences. d. pWX56 VIGS (15,798 bp), GFP gene inserted in-frame between NIb and CP coding sequences; and 249 bp each from target endogenous maize genes for magnesium chelatase (ZmChII), lemon white 1 (ZmIspH), and phytoene desaturase (ZmPDS). Black arrows indicate location of 5′ WX3 and 3′ MDMV-GenR1 primers (Table S1) used for amplification prior to in vitro transcription. RNA transcripts were synthesized from amplicon with primer-added 5′ T7 promoter sequence. P1 = protein 1 protease, HCPro = helper component protease, P3 = protein 3 protease. PIPO = pretty interesting Potyviridae open reading frame. 6k1 = 6 kD protein 1. Cl = cylindrical inclusions. 6 k2 = 6kD protein 2. VPg = viral protein genome linked. NIa-Pro = nuclear inclusion a-protease. NIb- RdRP = nuclear inclusion b-RNA dependent RNA polymerase. CP = coat protein