Fig. 5
From: Cold acclimation can specifically inhibit chlorophyll biosynthesis in young leaves of Pakchoi
Exogenous application of ALA can rescue the yellow leaves phenotype in cold-acclimated Y-05. a The leaf phenotype of ALA-treated plants compared with CK plants. ALA, cold-acclimated Y-05 sprayed by 1 mM ALA. CK, cold-acclimated Y-05 sprayed by water as control. Bar = 5 cm. b The chlorophyll content of ALA-treated plants was higher than CK plants. Chlorophyll a, Chlorophyll b and total chlorophyll abbreviated as Chl a, Chl b, Total Chl, respectively. c The ALA content of ALA-treatment plants was higher than CK plants. Three individual plants of each cultivar were quantified, and the chlorophyll and ALA content were measured three times. Error bars represent SE (±SE, n = 3). Different letters indicated statistically significant differences at the level of p < 0.05. d-k Observation of chloroplast ultrastructure showed that ALA-treated plants possessed mature chloroplasts and granum thylakoids. v = vacuole, gt = granum thylakoids, st = stroma thylakoids. In the Fig. 4d, f, h, j, bar = 2 μm; in the Fig. 4e, g, bar = 500 nm; in the Fig. 4i, k, bar = 800 nm