Fig. 1
From: Plant DNA methylation is sensitive to parent seed N content and influences the growth of rice
Nitrogen deficiency in S0 generation results in the decrease of plant height and yield of WYG7 and Ox1 and heritable in S1 generation in Ox1. (a, b) Gross morphology of WYG7 and Ox1 in +N and -N field in S0 generation. Bar = 20 cm. (c) Plant height and (d) Tiller number of WYG7 and Ox1 in +N and -N field in S0 generation. Error bar: SD (n = 10 plants). (e) Grain yield per plant of WYG7 and Ox1 in +N and -N field in S0 generation. Error bar: SD (n = 5 plants). (f) Relative expression of OsNAR2.1 in WYG7 and Ox1 in +N and -N field in S0 generation. Error bar: SD (n = 3 plants). (g) Plant height and (h) Tiller number of WYG7 and Ox1 in S1 generation. Error bar: SD (n = 10 plants). (i) Grain yield per plant of WYG7 and Ox1 in S1 generation. Error bar: SD (n = 5 plants). (j) Relative expression of OsNAR2.1 in WYG7 and Ox1 in S1 generation. Error bar: SD (n = 3 plants). (k) Plant height and (l) Tiller number of WYG7 and Ox1 in S2 generation. Error bar: SD (n = 10 plants). (m) Grain yield per plant of WYG7 and Ox1 in S2 generation Error bar: SD (n = 5 plants). (n) Relative expression of OsNAR2.1 in WYG7 and Ox1 in S2 generation. Error bar: SD (n = 3 plants). Significant differences between different lines are indicated by different letters (P < 0.05, one-way ANOVA). WYG7: wild type of Wuyungeng 7; Ox1: overexpression of OsNAR2.1 by OsNAR2.1 promoter; S0 + N: nitrogen treatment with 300 kg N/ha in S0 generation; S0-N: nitrogen treatment with 0 kg N/ha in the S0 generation