Fig. 6

Comparison of 5AL¹¹⁹, 5AL⁵³⁵, 5AL^{119 + 535}, and 5AL^No segments. a Model for the measurement of the segments on metaphase 5AL arms. Yellow bands indicate the signals of single-copy probes SC5A-479 and SC5A-527. Green bands indicate the signals of Oligo-pSc119.2–1. Red bands indicate the signals of Oligo-pTa535–1. ‘WL’ means the whole length of the 5AL arm. ‘MSL’ means the length of metaphase 5AL segment between SC5A-479 and SC5A-527 probes. b Comparison of relative metaphase length (RML) among the four kinds of segments in the four F₂ generations. c Comparison of RML among the four kinds of segments in the four wheat cultivars. Each dot represents the RML of an individual 5AL segment. The bars among the dots represent the mean values of RML. The number of 5AL segments (n) measured for each kind of segment is shown in parentheses. ns: no significant difference, **: p < 0.01, ***: p < 0.001