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Fig. 7 | BMC Plant Biology

Fig. 7

From: Identification of virulence associated milRNAs and their bidirectional targets in Rhizoctonia solani and maize during infection

Fig. 7

GRMZM2G412674 is attenuated expression by co-transforming with Rhi-milR-9829-5p and act as a positive regulator of maize BLSB. (a) The targeting sequence in the 3′ UTR region of GRMZM2G412674 by Rhi-milR-9829-5p and the mutation sites of f31 and f33 mutants. (b) Expression levels of GRMZM2G412674 assessed by the transient dual-luciferase assay. “GRMZM2G412674 (WT)” represents the predicted targeting sites, while “GRMZM2G412674 (Mut)” indicates the mutated target gene in the Rhi-milR-9829-5p binding sites. (c) Expression of GRMZM2G412674 in four-weeks B73, f31 and f33 lines using real-time RT-PCR. (d) Lesion length of the f31 and f33 mutant lines at 5 and 14 days after inoculation. (e) Expression patterns of GRMZM2G412674 detected by real-time RT-PCR. (f) Susceptible phenotype of the f31 and f33 mutant lines at 5 and 14 dpi. (g) Expression levels of Rhi-milR-9829-5p analyzed by real-time RT-PCR. 12 hpi, 24 hpi, 3 dpi, and 5 dpi indicate 0 h, 12 h, 24 h, 3d, and 5d post inoculation, respectively. The bars represent the mean ± SD. The asterisk indicates a significant difference with P-value < 0.05

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