Fig. 8
Fluorescent microscopic images of P. syringae pv. tabaci bacteria treated with an AS mix, and stained to evaluate changes in membrane permeability and polarity. P. syringae pv. tabaci (105 CFU/ml) was treated with a mixture of AS + H2O2 + POX. After 3 h of shaking, (a) DIBAC4(3), an indicator dye of membrane depolarization or (b) SYTOX Green, an indicator of membrane permeability, and DAPI as counterstain were added and co-incubated for 1 h. After concentration of the bacteria, microscopic slides were prepared and photographed within 1–2 h. Bar = 100 μm. Abbreviations: Control: untreated bacteria AS: acetosyringone, HK: heat-killed, POX: horseradish peroxidase