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Table 2 The effect of BBM and WUS on Agrobacterium-mediated barley transformation using immature embryos as explants

From: Identification of regulatory factors promoting embryogenic callus formation in barley through transcriptome analysis

Developmental Gene

Expression Cassettes

No. of

explants

No. of regenerated plants

Regeneration

Freq.

No. of T0

Transformation Freq.

pCAMBIA1305 EV

615

48

7.80%

45

7.32%

proZmPLTP:ZmBBM

+proZmAxig1:ZmWUS

575

27

4.7%

23

4.00%

proZmPLTP:HvBBM

+proZmAxig1:HvWUS

613

155

25.29%

152

24.8%

  1. Agrobacterium (strain EHA105)-mediated barley transformation was performed using immature embryos as explants. The proZmPLTP:HvBBM + proZmAxig1:HvWUS construct contains two cassettes, the first of which includes a maize phospholipid transferase promoter (proZmPLTP) driving HvBBM, and the second includes the maize Axig1 promoter (proZmAxig1) driving the HvWUS. The pCAMBIA1305 empty vector (EV) was used as a control. Regeneration Freq. was estimated as the No. of regenerated plants divided by the No. of the explants, and the transformation frequency was calculated as No. of T0 divided by No. of explants