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Fig. 7 | BMC Plant Biology

Fig. 7

From: Identification of regulatory factors promoting embryogenic callus formation in barley through transcriptome analysis

Fig. 7

The effect of BBM and WUS ectopic expression on callus-inducing medium (CIM)-induced callus formation and transformation efficiency. a Schematic representation of the construct used for Agrobacterium-mediated barley transformation. The proZmPLTP:HvBBM + proZmAxig1:HvWUS construct contained two cassettes: the first one included the maize phospholipid transferase promoter (proZmPLTP) driving HvBBM with a Nos terminator, and the second one included the maize Axig1 promoter (proZmAxig1) driving HvWUS with a Nos terminator. b The callus-forming and plant regeneration phenotype after Agrobacterium inoculation. The group using an empty vector was set as control. c Fresh weight analysis of callus in the control and proZmPLTP:HvBBM + proZmAxig1:HvWUS transformation group. Error bars indicate the SE of the mean (n = 30). The experiments were performed in three independent replicates. d The effect of BBM and WUS ectopic expression on the genes in the LEC1-ABI3-FUS3-LEC2 network. The data shown are means ± S.D. of three biological replicates.**, P < 0.05; ***, P <0.01 (Student’s t-test).

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