Fig. 4

ABA response of some ABA signaling component genes in the Col wild type and the aitr256, aitr1256 and aitr123456 mutants. a Fold changs of the PYR/PYL receptor gene PYL4, PYL5 and PYL6. b Fold changs of PP2C phosphatase gene HAI1 and ABF transcription factor gene ABF3. c Fold changs of SnRK2 kinase gene SnRK2.2, SnRK2.3 and SnRK2.6. Twelve-day-old seedlings of the Col wild type and the aitr256, aitr1256 and aitr123456 mutants were treated with 50 µM ABA, total RNA was isolated and quantitative RT-PCR was used to examine the expression of key regulator genes in ABA signaling. The expression of ACT2 was used as an inner control, and the fold changes were calculated by comparing the expression levels of the corresponding genes in ABA treated and un-treated samples. The experiments were repeated three times, and similar results were obtained. Data represent the mean ± SD of three replicates