Fig. 2

Effects of ABA on seed germination of the aitr mutants. Seeds of the Col wild type, the aitr256 triple, aitr1256 quadruple, aitr23456 quintuple and aitr123456 sextuple mutants were sterilized and sown on 1/2 MS plates in the presence or absence of 1 µM (up panel) or 2 µM ABA (low panel). The plates were transferred, after kept at 4 °C in darkness for 2 days, to a growth room. All the seeds on plates in the absence of ABA were germinated one day after the transfer. Seed germination on ABA-containing plates was examined daily after the transfer, germinated seeds were scored, and percentage of germination was calculated. The experiments were repeated three times, and similar results were obtained. Data represent the mean ± SD of three replicates