Fig. 4

The identification and analysis of MELO3C025758. a The multiple-effect QTL for fruit weight and fruit diameter in WAP (‘JL475’ × ‘YS474’). b Distribution of nucleotide diversity (π) of wild and cultivated agrestis around the bin855. c Phylogenetic tree of MELO3C025758 and its orthologues in rice (Oryza sativa), Arabidopsis (Arabidopsis thaliana), tomato (Solanum lycopersicum), potato (Solanu tuberosum), pumpkin (Cucurbita moschata), watermelon (Citrullus lanatus) and cucumber (Cucumis sativus). The bootstrap value (100 replications) was listed on the main clades. d Gene structure of MELO3C025758, line means intron, rectangle means exon and the short red line indicates the position of two adjoint SNPs. Genotyping of the non-synonymous substitution of MELO3C025758 in six dicots. The genes listed out are all the best hit genes of MELO3C025758 (Cucumis sativus: CsaV3_6G021720, Citrullus lanatus: Cla000557, Cucurbita moschata: CmoCh20G005090, Cucurbita moschata: CmoCh02G008800, Arabidopsis thaliana: AT5G60450, Solanum lycopersicum: Solyc11g069190, Solanum tuberosum: PGSC0003DMC400014194, Solanum tuberosum: PGSC0003DMC400014193). e qRT-PCR analysis of MELO3C025758 in flesh tissue (15 days after pollination), C1-C6 and W1-W3 represent the cultivated and wild accessions, respectively. The distribution of fruit weight (f) and fruit diameter (g) with three different genotypes that composed by two adjoint SNPs (CA, GC and SM, in which SM means both two SNPs are heterozygous) in WAP, each box represents the mean and interquartile range. P-value was marked (T-test, two tail)