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Fig. 6 | BMC Plant Biology

Fig. 6

From: Structural and functional analysis of tomato sterol C22 desaturase

Fig. 6

Test of C22DESΔ2–27 enzymatic activity in vivo (a) Cropped image from immunoblot analysis of C22DES-GFP (≈87.24 kDa) and C22DESΔ2–27-GFP (≈84.22 kDa) of agroinfiltrated N. benthamiana leaves. Full-length blot is presented in Additional file 8: Fig. S6. b Stigmasterol levels in total sterol fractions of N. benthamiana leaves expressing C22DES-GFP and C22DESΔ2–27-GFP. Values are mean values ± SD of three technical replicates (n = 3). Lowercase letters indicate significant differences among mean values relative to those in leaf samples expressing the empty vector (one-way ANOVA with Dunnett’s multiple comparisons test)

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