Fig. 5

Targeting and retention of the C22DES globular domain in the ER. a Confocal optical sections showing the fluorescence of C22DESΔ2–27-RFP (left) and TMH-GFP (middle) transiently expressed in N. benthamiana leaves. Merged images are shown on the right. b FRAP curves representing the fluorescence recovery rates of C22DESΔ2–27-RFP, BRL3-GFP, and GFP. Fluorescence recovery curves represent the best fits from normalized datasets of at least 6 independently bleached points spots. (C) Cropped images of immunoblot analysis of soluble (S) and membrane (M) cell fractions from N. benthamiana leaves transiently expressing BRL3-GFP (≈153 kDa) and GFP (≈26.8 kDa) as membrane bound and soluble control proteins, respectively. Full-length blots are presented in Additional file 6: Fig. S4. c Cropped images from immunoblot analysis of soluble (S) and membrane (M) cell fractions from N. benthamiana leaves transiently expressing C22DESΔ2–27-GFP (≈84.2 kDa), TMH-GFP (≈34 kDa), and C22DES-GFP (≈87.2 kDa). Full-length blots are shown in Additional file 7: Fig. S5