Fig. 4

Role of TMH1 in the targeting and retention of C22DES in the ER membrane. a Schematic representation of the GFP fusion constructs generated to study the role of TMH and MCR1 in the targeting and retention of C22DES in the ER. Grey boxes indicate the transmembrane helix (TMH), orange boxes correspond to the MCR1 motif and green boxes correspond to the GFP protein. The amino acid sequence of TMH and MCR1 are shown below the corresponding regions. b Confocal optical sections showing the GFP fluorescence pattern of N. benthamiana cells transiently expressing TMH + MCR1-GFP, TMH-GFP, and MCR1-GFP. The arrow indicates the cell nucleus (n). c Close-up view of the fluorescence pattern of TMH-GFP and MCR1-GFP (left), T3RE (middle) and the corresponding merged images (right)