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Fig. 1 | BMC Plant Biology

Fig. 1

From: Characterization of chromosome constitution in three wheat - Thinopyrum intermedium amphiploids revealed frequent rearrangement of alien and wheat chromosomes

Fig. 1

GISH, FISH and McGISH patterns of TE261–1, TE266–1, TE346–1 and YN15. GISH patterns of TE261–1 (A1), TE266–1 (B1) and TE346–1 (C1): St (Ps. strigosa)-genome DNA labeled with Texas-Red-5-dCTP was used as the probe, and YN15 genome DNA was employed as the block. FISH patterns of TE261–1 (A2), TE266–1 (B2), TE346–1 (C2) and YN15 (D1): red signals represent (GAA)8 labeled with 5′ TAMRA, and green signals represent pAs1 labeled with fluorescein-12-dUTP. McGISH patterns of TE261–1 (A3), TE266–1 (B3), TE346–1 (C3) and YN15 (D2): A genomes are labeled with fluorescein-12-dUTP; D genomes are labeled with Texas-Red-5-dCTP; and B-genome DNA (gray) is used for blocking. A-D (7D) translocation are indicated by red asterisks in McGISH patterns (A3, B3 and C3); A-D (2A) translocation are indicated by yellow asterisks in TE266–1 (B3); and 2A, 2D and 7D are marked in the McGISH patterns of YN15 (D2)

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