Fig. 1From: Characterization of chromosome constitution in three wheat - Thinopyrum intermedium amphiploids revealed frequent rearrangement of alien and wheat chromosomesGISH, FISH and McGISH patterns of TE261–1, TE266–1, TE346–1 and YN15. GISH patterns of TE261–1 (A1), TE266–1 (B1) and TE346–1 (C1): St (Ps. strigosa)-genome DNA labeled with Texas-Red-5-dCTP was used as the probe, and YN15 genome DNA was employed as the block. FISH patterns of TE261–1 (A2), TE266–1 (B2), TE346–1 (C2) and YN15 (D1): red signals represent (GAA)8 labeled with 5′ TAMRA, and green signals represent pAs1 labeled with fluorescein-12-dUTP. McGISH patterns of TE261–1 (A3), TE266–1 (B3), TE346–1 (C3) and YN15 (D2): A genomes are labeled with fluorescein-12-dUTP; D genomes are labeled with Texas-Red-5-dCTP; and B-genome DNA (gray) is used for blocking. A-D (7D) translocation are indicated by red asterisks in McGISH patterns (A3, B3 and C3); A-D (2A) translocation are indicated by yellow asterisks in TE266–1 (B3); and 2A, 2D and 7D are marked in the McGISH patterns of YN15 (D2)Back to article page