Fig. 4
From: Loss of mature D1 leads to compromised CP43 assembly in Arabidopsis thaliana
The C-terminal extension of pD1 hindered the interaction of D1 with RC complex proteins in yeast two-hybrid analysis. a Yeast two-hybrid analysis of the interactions between mature D1 / pD1 and RC complex proteins. Yeast cells expressing D1-Cub or pD1-Cub with various NubG-X (CP43, CP47 and D2) constructs were grown to logarithmic phase (OD600 = 0.5), and 5 μl portions of 1:10 serial dilutions were spotted on SD-Trp-Leu plates, and SD-Trp-Leu-His-Ade plates without or with 2 mM 3-AT, and incubated at 30 °C for 2 days. pDSL-Nx vector was used as the negative control, and NubI was used as the positive control. b Growth curves of yeast strains cultured in SD-Trp-Leu-His-Ade liquid medium containing 30 μg / ml kanamycine. Cells were grown at 30 °C with 200 rpm for the indicated period. Statistical analysis was shown in Tab. S2. Different letters indicated a significant difference among different values. Duncan’s multiple range test, p ≤ 0.05, n = 6