Fig. 5

Effect of salt stress on the expression of genes involved in the L-gal pathway of ascorbic acid (AsA) synthesis and encoding ascorbic acid peroxidase (APX). The gene expressions of VTC1, VTC4, VTC5, GaIDH, GLDH, APX1, APX2, APX3, APX4, APX5, and APX6 in Col-0 seedlings under 200 mM NaCl were analyzed by qPCR. After normalizing to the internal control ACTIN, the transcript levels at indicated treatment times were compared with that at 0 h, which was normalized as “1”. Error bars represent the SD from three biological replicates. Statistically significant differences are indicated by star symbol (P < 0.05, Mann-Whitney U test). 7-day-old seedlings were treated with 200 mM NaCl for 24 h