Fig. 3

ABI4 confers to ascorbic acid (AsA) biosynthesis and reactive oxygen species (ROS) accumulation under salt stress. a AsA contents in Col-0, abi4–102, abi4–103 and ABI4-overexpressing lines under the 100 mM NaCl treatment. b Diaminobenzidine (DAB) and nitroblue tetrazolium (NBT) staining of ROS in Col-0, abi4–102, abi4–103 and ABI4-overexpressing seedlings with or without the salt treatment. Measurements of c H₂O₂ and d O₂⁻ in Col-0, abi4–102, abi4–103 and ABI4-overexpressing lines with or without the salt treatment. 7-day-old seedlings were treated with 100 mM NaCl (with or without 40 μM AsA) for 24 h. Values in a, c and d are means ± SD (n = 3). Statistically significant differences are indicated by different letters (P < 0.05, ANOVA with Tukey’s test). FW, fresh weight