Transcriptional changes of key flowering regulator genes in the Ehd1-Hd3a/RFT1 pathway induced by overexpression of MBD707. a. Heatmap showing the differential expression levels of flowering genes in the OsMBD707-overexpression line (OX707-#21) and wild-type (NPB) revealed by RNA-seq. Grey blocks indicate that the genes were not detected as differentially expressed genes (DEGs) by RNA-seq. b. Schematic model showing the differentially expressed flowering genes involved in the Ehd1-Hd3a/RFT1 regulatory network. Red and green backgrounds indicate that the genes were down-regulated and up-regulated, respectively, in OX707-#21. Yellow background represents that the down-regulation of DTH2 was paradoxical to the early flowering phenotype of OX707-#21. Grey backgrounds represent that the genes were not detected as DEGs by RNA-seq. Black arrows indicate a promoting effect, bars indicate a repressive effect, and dotted lines indicate an unknown pathway. c. qRT-PCR validation of five key flowering regulator genes Ehd1, Hd3a, RFT1, OsMADS14, and OsMADS15 that were identified as DEGs between OX707-#21 and NPB by RNA-seq. The frameshift mutant line mbd707-#6 was also included in the experiment. The rice Actin gene was used as an internal control.