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Fig. 4 | BMC Plant Biology

Fig. 4

From: Overexpression of a methyl-CpG-binding protein gene OsMBD707 leads to larger tiller angles and reduced photoperiod sensitivity in rice

Fig. 4

Molecular characterization of OsMBD707-overexpression, −knockdown and -frameshiftplants. a, b. qRT-PCR analysis of the transcript levels of OsMBD707 in rice plants transformed with OsMBD707-overexpression construct (OX707-#8, OX707-#14, OX707-#20, OX707-#21, OX707-#40) and OsMBD707-RNAi construct (707i-#11, 707i-#22, 707i-#26, 707i-#30, 707i-#39), respectively. NPB, nontransgenic wild-type Nipponbare. The rice Actingene was used as an internal control. c. Schematic illustration of the OsMBD707 gene structure and mutations of OsMBD707 in CRISPR/Cas9-mediatedmutated plants. Black rectangles represent the 2 exons of OsMBD707; Red characters indicate the sequences of the target sites; PAM sequences are underlined; Deletions are indicated by dashes; Numbers on the right side indicate the numbers of deletion nucleotides; WT indicates wild-type sequence

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