Fig. 3From: Overexpression of a methyl-CpG-binding protein gene OsMBD707 leads to larger tiller angles and reduced photoperiod sensitivity in riceExpression pattern of OsMBD707 and subcellular localization of OsMBD707. a. Histochemical GUS staining of the roots, stems, leaves, and spikelets of rice plant transformed with the OsMBD707 promoter-GUS fusion construct. NPB, nontransgenic wild-type Nipponbare; p35S-GUS, rice plant transformed with a CaMV 35S promoter-GUS construct; pMBD707-GUS, riceplant transformed with the OsMBD707 promoter-GUS construct. Scale bar, 2 mm. b. Subcellular localization of the GFP-OsMBD707 fusion in rice protoplasts. 35S-GFP + DsRed-OsH2B, rice protoplast co-transfected with a GFP alone construct and a nucleus marker construct DsRed-OsH2B; GFP-OsMBD707 + DsRed-OsH2B, rice protoplast co-transfected with the GFP-OsMBD707 fusion construct and DsRed-OsH2B; Bright, imagestaken under bright field; GFP, images taken under green fluorescence; RFP, images taken under red fluorescence; Merged, images merged from bright filed and green fluorescence channels. Scale bar, 10 μmBack to article page