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Fig. 3 | BMC Plant Biology

Fig. 3

From: Overexpression of a methyl-CpG-binding protein gene OsMBD707 leads to larger tiller angles and reduced photoperiod sensitivity in rice

Fig. 3

Expression pattern of OsMBD707 and subcellular localization of OsMBD707. a. Histochemical GUS staining of the roots, stems, leaves, and spikelets of rice plant transformed with the OsMBD707 promoter-GUS fusion construct. NPB, nontransgenic wild-type Nipponbare; p35S-GUS, rice plant transformed with a CaMV 35S promoter-GUS construct; pMBD707-GUS, riceplant transformed with the OsMBD707 promoter-GUS construct. Scale bar, 2 mm. b. Subcellular localization of the GFP-OsMBD707 fusion in rice protoplasts. 35S-GFP + DsRed-OsH2B, rice protoplast co-transfected with a GFP alone construct and a nucleus marker construct DsRed-OsH2B; GFP-OsMBD707 + DsRed-OsH2B, rice protoplast co-transfected with the GFP-OsMBD707 fusion construct and DsRed-OsH2B; Bright, imagestaken under bright field; GFP, images taken under green fluorescence; RFP, images taken under red fluorescence; Merged, images merged from bright filed and green fluorescence channels. Scale bar, 10 μm

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