Fig. 5
From: MaMAPK3-MaICE1-MaPOD P7 pathway, a positive regulator of cold tolerance in banana
(A) Physical interactions between MaICE1 and MaPOD P7 using Y2H assays. (B) Biomolecular fluorescence complementation visualization of the interaction betweenMaICE1 and MaPOD7. In the BiFC assay, MaICE1 and MaPOD7 DNA fragments were fused with the split mVenus N-terminal and C-terminal in the vectors of pRTVnVN and pRTVnVC, which the marker genes ECFP and mCherry were added into the pRTVnVN and pRTVnVC vectors, respectively. After co-transfection, we detected clear yellow fluorescence signals on the nucleus of the transfected protoplasts, indicating the formation of reconstituted mVenus through the MaICE1-MaPOD interaction