Fig. 4
From: MaMAPK3-MaICE1-MaPOD P7 pathway, a positive regulator of cold tolerance in banana
Cold tolerance assay of transgenic Cavendish banana plants. (A) Phenotypes of transgenic Cavendish banana plants (#11 and #13), 'Dajiao' and the wild-type (WT) before and after cold treatment (10 °C for 48 h), followed by recovery in an ambient environment for 3 days (B) Injured area of WT banana and transgenic plants analyzed after cold treatment (bar: 3.5 cm). Effect of MaICE1 expression in banana on levels of (C) electrolyte leakage, (D) pro and (E) MDA. Two-month-old banana plants of WT, ‘Dajiao’ and transgenic Cavendish banana plants (#11 and #13) were exposed to 10 °C for 48 h. Leaves were collected before and after cold treatment. FW, fresh weight. Each value represents the means of three biological replicates, and vertical bars indicate the SE. **P < 0.01. ***P < 0.001