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Fig. 1 | BMC Plant Biology

Fig. 1

From: Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae

Fig. 1

Multiple sequence alignments of plant, mammal and insect SDF2-type proteins. The sequences were aligned with Clustal Omega tool, using default settings (EMBL-EBI; https://www.ebi.ac.uk/Tools/msa/). For a better alignment, signal peptides predicted with ‘SignalP 4.1 Server’ (http://www.cbs.dtu.dk/services/SignalP/) [23] were removed from the sequences. The sequence of Plutella xylostella PxSDF2L1 has been published (GenBank accession no. HQ199329). The other SDF2 sequences are coded by their GenBank accession no. followed by the abbreviated name of the organism: A.t., Arabidopsis thaliana; N.a., Nicotiana attenuata, H.s., Homo sapiens; M.m.: Mus musculus; T.c., Tribolium castaneum; B.m., Bombyx mori. In the alignment, the stretch of amino acid residues encompassing each MIR motif has been highlighted in different colours: MIR1 in magenta, MIR2 in cyan, and MIR3 in green. Conserved residues at the interior of the β-trefoil barrel arranged in the bottom and middle layers are squared in black and blue, respectively. The key residues of the triangular cap are squared in red. The ER retention tetrapeptides ‘HDEL’ or ‘HTEL’ [24] appear in red

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